Precise tissue assembly using avidin-biotin binding system and optical tweezers
نویسندگان
چکیده
We have been investigating the feasibility of bottom-up tissue engineering, a new methodology to form tissues, by assembling single cells one by one using an avidin-biotin binding system (ABBS). In this report, we used optical tweezers in addition to ABBS to form small tissues where the cells alignment was precisely controlled. ABBS enabled very quick attachment of human hepatoma Hep G2 cells, and single cell manipulation by optical tweezers aligned the cells accurately. These results show that the combination of two technologies, ABBS and single cell manipulation, provides a new methodology for bottom-up tissue engineering.
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